Recombinant Aquaporin 4 antibody (Extracellular Domain)

Catalog No. ABIN7543244

This Mouse Monoclonal antibody specifically detects Aquaporin 4 in IP, ELISA, FACS and InhA. It exhibits reactivity toward Human.

Quick Overview for Recombinant Aquaporin 4 antibody (Extracellular Domain) (ABIN7543244)

Target: Aquaporin 4 (AQP4)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aquaporin 4 antibody is un-conjugated

Application: Immunoprecipitation (IP), ELISA, Flow Cytometry (FACS), Inhibition Assay (InhA)

Clone: C9401

Product Details anti-Aquaporin 4 Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-Aquaporin 4 [C9401], Mouse IgG2b, kappa

Specificity:

This antibody recognizes and binds extracellular domains of both the M1 and M23 isoforms of human aquaporin 4 (AQP4), but not mouse aquaporin 4. AQP4 is a water channel expressed on astrocytes throughout the CNS and in skeletal muscle and some epithelial cells outside of the CNS. It plays an important role in brain water homeostasis and in glymphatic solute transport. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system (CNS) that is associated with demyelination and neurological deficits in optic nerve, spinal cord and brainstem. Most NMOSD patients are seropositive for IgG autoantibodies (NMO-IgG) directed against extracellular epitopes of aquaporin-4 (AQP4). Prevention of the binding of NMO-IgG, therefore, may alleviate the disease.

Application Notes original Clone: This antibody binds extracellular domain of human aquaporin 4 with a very high binding affinity. The binding of this antibody inhibits the binding of NMO-IgG to AQP4 (PMID: 23746426). The binding affinity of this antibody for human AQP4 M23 isoform expressed on the surface of baculovirus and CHO cells was tested with ELISA (PMID: 26398585, WO2014196658). The binding characterization of this antibody was done using flow cytometry. This antibody can be used for the immunoprecipitation of human AQP4 but not mouse AQP4 (WO2014196658, PMID: 23909421). This antibody is not suitable for detection of denatured human AQP4 in a western blot assay (WO2014196658).

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing an aquaporin 4 knock out mice with human AQP4 as an immunogen expressed on the surface of baculovirus.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2b

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Aquaporin 4

Target: Aquaporin 4 (AQP4)

Alternative Name: Aquaporin 4

Background: AQP4, AQP-4, Aquaporin-4, MIWC, WCH4, Mercurial-insensitive water channel

UniProt: P55087

Pathways: Sensory Perception of Sound