Recombinant CD47 antibody (Extracellular Domain)

Catalog No. ABIN7543262

This Rabbit Chimeric antibody specifically detects CD47 in FACS and ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD47 antibody (Extracellular Domain) (ABIN7543262)

Target: CD47

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD47 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA

Clone: 2D14

Product Details anti-CD47 Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-CD47 [2D14], Rabbit IgG, kappa

Specificity: This antibody binds the extracellular domain of the human CD47, which is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epithelia and the brain. IAP or integrin assocaited protein has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It further plays an important role in memory formation and synaptic plasticity in the hippocampus. It also acts as a receptor for SIRPA. Binding to SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with recombinant human CD47 extracellular domain with equal volume of adjuvant as an immunogen.

Isotype: IgG kappa

Application Details

Application Notes: The binding specificity of this antibody for recombinant human CD47 was done using ELISA. This antibody is capable of detecting CD47 expressed on the cell surface of K562 (human chronic myeloid leukemia cells) in a flow cytometry (CN110066336).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: IAP, MER6, OV-3, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, ovarian cancer marker

UniProt: Q08722