Recombinant F2RL1 antibody (Cleavage Site)

Catalog No. ABIN7543275

The Mouse Monoclonal anti-F2RL1 antibody is suitable to detect F2RL1 in samples from Human. It has been validated for ELISA.

Quick Overview for Recombinant F2RL1 antibody (Cleavage Site) (ABIN7543275)

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This F2RL1 antibody is un-conjugated

Application: ELISA

Clone: 1B5

Product Details anti-F2RL1 Antibody

Binding Specificity: Cleavage Site

Purpose: Anti-PAR2 [1B5], Mouse IgG1, lambda

Specificity:

This antibody binds human PAR2 or proteinase-activated receptor 2 upstream to its cleavage site. PAR2 acts as a receptor for trypsin and trypsin-like enzymes coupled to G proteins.

Application Notes original Clone: The initial screening of this antibody for reactivity for PAR2 was done using ELISA. The equilibrium dissociation constant (KD) of this antibody was obtained using KinExA® software and by ""n-curve analysis"" and it was reported that the original antibody had a KD of 3.39 nM with a 95 % confidence interval of 1.36 to 5.47 nM (US8357367).

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing XenoMouse™ with PAR-2 antigen.

Isotype: IgG1, lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for F2RL1

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Alternative Name: PAR2

Background: F2RL1, PAR-2, GPR11, Proteinase-activated receptor 2, Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like 1

UniProt: P55085

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome