Recombinant BCMA antibody

Catalog No. ABIN7543279

The Mouse Monoclonal anti-BCMA antibody (Clone CA8) (ABIN7543279) specifically detects BCMA in ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for Recombinant BCMA antibody (ABIN7543279)

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCMA antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Clone: CA8

Product Details anti-BCMA Antibody

Purpose: Anti-BCMA [CA8], Mouse IgG1, kappa

Specificity: This antibody binds human and cynomolgus monkey BCMA and does not bind TACI or BAFF-R proteins. BCMA acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. BCMA expression is highest on terminally differentiated B cells. BCMA is involved in mediating the survival of plasma cells for maintaining long-term humoral immunity. It also activates NF-kappa-B and JNK. The expression of BCMA has been linked to a number of cancers, autoimmune disorders, and infectious diseases.

Cross-Reactivity: Cynomolgus

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original mouse parental antibody 118G03 was generated by immunizing SJL mice with recombinant human BCMA/TNFRSF17-Fc chimera (R&D 193-Fc) by using the RIMMS method (Rapid immunization multiple sites). A chimeric version of this antibody was generated and it is called CA8.

Isotype: IgG1 kappa

Application Details

Application Notes: The binding characterization of this antibody towards human and cynomolgus monkey recombinant BCMA was done using ELISA (US9273141). The binding of the antibody to membrane bound BCMA expressed on HEK293, H929, OPM-2, JJN-3 and U266 cells was done using flow cytometry (US9273141). This antibody neutralizes binding of both BCMA ligands BAFF and APRIL, and subsequently blocks the induction of NF-κB signaling cascades (PMID: 26370838). The EC50 values for chimeric CA8 were calculated to be 0.695 μg/mL and 0.773 μg/mL respectively (US9273141). This antibody neutralized both BAFF and APRIL induced NfkappaB cell signaling in H-929 cells. This antibody was also reported to kill BCMA expressing ARH77 cells via ADCC. This antibody was also used in the generation of an antibody-drug conjugate for possible application in the treatment of multiple myeloma (US9273141).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for BCMA

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Alternative Name: BCMA

Background: CD269, BCM, TNFRSF17, Tumor necrosis factor receptor superfamily member 17, B-cell maturation protein, S307118G03, 118G03

UniProt: Q02224