Recombinant Insulin antibody

Catalog No. ABIN7543284

The Rabbit Chimeric anti-Insulin antibody has been validated for ELISA, FACS and RIA. It is suitable to detect Insulin in samples from Cow.

Quick Overview for Recombinant Insulin antibody (ABIN7543284)

Target: Insulin (INS)

Antibody Type: Recombinant Antibody

Reactivity: Cow

Host: Rabbit

Clonality: Chimeric

Conjugate: This Insulin antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Radioimmunoassay (RIA)

Clone: Ab 123

Product Details anti-Insulin Antibody

Purpose: Anti-Insulin [Ab 123 (mAb3, CC9C10)], Rabbit IgG, kappa

Specificity: This antibody binds an epitope on the B chain of calf, human and rodent insulin. Insulin is a peptide hormone produced by the beta cells of the pancreatic islets. It reduces blood glucose concentration by promoting its absorption from the blood into liver, fat and skeletal muscle cells. Decreased or absent insulin activity results in diabetes mellitus, a condition of high blood sugar level (hyperglycemia).

Cross-Reactivity: Human, Rodents

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing female BALB/c mice with calf insulin in Freund's complete adjuvant.

Isotype: IgG kappa

Application Details

Application Notes: This antibody can be used to detect insulin in a sample using ELISA and radioimmunoassay (PMID: 2109009, 6193965). This antibody was used in the flow cytometric analysis of insulin occupied B cell receptors in VH125SD B6 mice (PMID: 26109644).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: INS, proinsulin

UniProt: P01317

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation