Recombinant Actin antibody

Catalog No. ABIN7543296

The Mouse Monoclonal anti-Actin antibody has been validated for ELISA and IHC (p). It is suitable to detect Actin in samples from Human.

Quick Overview for Recombinant Actin antibody (ABIN7543296)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3G8C7A6

Product Details anti-Actin Antibody

Purpose: Anti-Alpha actin 1 [3G8C7A6], Mouse IgG2b, kappa

Specificity: This antibody binds actin. Actin is a type of spherical multi-functional protein that forms a microfilament. Actin is widely existent in eukaryotic organisms, and there is a major component constituting the muscle segment and the cell skeleton in the cytoplasm and cellular nucleus of the intramuscular cell. Muscle specific actin (MSA) is a highly conserved, ubiquitous protein found in muscle and some non-muscle cells. In addition to participating in the normal morphology of maintaining cells, actin also participates in the formation, adhesion, migration, apoptosis, material transport, transmembrane information transfer, and receptor aggregation.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with a recombinant human actin protein.

Isotype: IgG2b kappa

Application Details

Application Notes: This antibody is highly similar to the commercially available anti-actin antibody HHF35. This antibody can use used in the immunohistochemical staining of smooth fibroids. This antibody can be used in the identification of actin from various tissues including brain, heart, cerebellum, esophageal, adrenal, stomach, ovaries, small intestines, pancreatic, colorectal, parathyroid, liver, pituitary, salivary gland, testicular, kidney, thyroid, prostate, breast, uterus, spleen, bladder, tonsil, skeletal muscle, thymus (young children), skin, bone marrow, peripheral nerve, lung, melodder cells. The binding specificity of this antibody towards actin was determined using ELISA. The original IgG2b antibody binds actin with an affinity constant of 6.26X10^9 M^(-1) (CN113817055).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: Alpha actin 1

Background: Actin, ACTA1, ACTA, Actin alpha skeletal muscle, Alpha-actin-1, Muscle specific actin, MSA, HHF35, CGMCNO.22312, HHF35

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development