Recombinant ITGA4 antibody

Catalog No. ABIN7543376

This Rabbit Chimeric antibody specifically detects ITGA4 in FACS, IP, BR and InhA. It exhibits reactivity toward Human and Guinea Pig.

Quick Overview for Recombinant ITGA4 antibody (ABIN7543376)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Guinea Pig

Host: Rabbit

Clonality: Chimeric

Conjugate: This ITGA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR), Inhibition Assay (InhA)

Clone: HP1-2

Product Details anti-ITGA4 Antibody

Purpose: Anti-Integrin alpha 4 [HP1/2], Rabbit IgG, kappa

Specificity:

This antibody specifically binds epitope B of the alpha 4 chain of human integrin alpha 4 (CD49d). Integrins alpha-4/beta-1 (VLA-4) acts as a receptor for fibronectin and VCAM1. VLA-4 is atypical because it is mostly restricted to lymphoid and myeloid cells, and indirect evidence had suggested that it might be involved in various cell-cell interactions. In addition, VLA-4 has been shown to mediate T and B lymphocyte attachment to the heparin II binding fragment of human plasma fibronectin (FN). This antibody is also reported to cross react with integrin alpha-4/beta-7 (α4β7).

Application Notes original Clone: This antibody can be used for the immunoprecipitation of VLA-4 from cell lysates (PMID: 2430809). This antibody is also capable of detecting integrin alpha 4 expressed on the surface of Ramos cells by flow cytometry (PMID: 2430809, 1689216). This antibody can also block adherence of CTT leukocytes to recombinant soluble VCAM 1-coated plates in vitro (PMID: 7686922). This antibody can also block the interaction of VLA-4 with fibronectin. This antibody does not cause cell aggregation (PMID: 1709929). This antibody can bind soluble integrin alpha-4 in an ELISA. This antibody also inhibited the binding of PAF-stimulated eosinophils to MAdCAM-1 transfectants. This antibody also inhibited binding of unstimulated eosinophils to soluble recombinant human (rh)VCAM-1 and fibronectin (Fn) (PMID: 8911148).

Cross-Reactivity: Guinea Pig

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Purification: Protein A affinity purified

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: Integrin alpha 4

Background: CD49d, VLA-4, α4, VLA4, ITGA4, α4β1, α4 integrin, VLA4 heterodimer (α4β1), Integrin alpha-4, Integrin alpha-IV, VLA-4 subunit alpha, CD49 antigen-like family member D, B1 integrin very late activation antigen-4

UniProt: P13612

Pathways: Integrin Complex