Recombinant HLA-E antibody

Catalog No. ABIN7543434

The Rabbit Monoclonal anti-HLA-E antibody has been validated for FACS, IF and Func. It is suitable to detect HLA-E in samples from Human.

Quick Overview for Recombinant HLA-E antibody (ABIN7543434)

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HLA-E antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: 3D12

Product Details anti-HLA-E Antibody

Purpose: Anti-HLA-E [3D12], Rabbit IgG, kappa

Specificity: This antibody binds an extracellular epitope on the human HLA-E, a member of human leukocyte antigen family. This antibody is capable of reacting with free HLA-E heavy chain as well as heavy chain associated with b2-microglobulin and peptide. This antibody is reported to cross react with HLA-B and HLA-C but not with HLA-A, HLA-F or HLA-G. The human HLA-E is a non-classical MHC class 1B molecule involved in immune self-nonself discrimination. HLA-E plays a very important role in cell recognition by NK cells. Peptide-bound HLA-E-B2M heterotrimeric complex primarily functions as a ligand for natural killer (NK) cell inhibitory receptor KLRD1-KLRC1, enabling NK cells to monitor the expression of other MHC class I molecules in healthy cells and to tolerate self.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing HLA-B27 transgenic mice with recombinant human HLA-E purified from AEH cells (Lymphoblastoid cell line LCL 721.221 cells transfected with HLA-E gene).

Isotype: IgG kappa

Application Details

Application Notes: The biotin conjugated version of this antibody was used for the study of HLA-E expression and distribution on PBMCs using flow cytometry. This antibody was also used in an assay to study the effect of HLA-E on inhibition of NKL-mediated cytolysis LCL .221 cell line. The presence of 3D11 the resulted in complete restoration of lysis (PMID: 9560253). It was reported that binding of this antibody to HLA-E is inhibited by the peptides sequences 115QFAYDGKDY123 and 137DTAAQI142. It was also reported that this antibody is capable of binding several HLA-Ia antigens like HLA-B and HLA-C (PMID: 21145594). This antibody was used in a study to determined the surface expression of HLA-E in human tumors using immunofluorescent staining and flow cytometry (PMID: 12618909).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for HLA-E

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Alternative Name: HLA-E

Background: HLAE, sHLA-E, HLA-6.2, HLA class I histocompatibility antigen alpha chain E, Major histocompatibility complex class I E, MHC, MHC class I antigen E, MHC HLA E alpha 1, MHC HLA E alpha 2.1, HLAB, HLA-B, HLA-C, HLAC

UniProt: P13747, P01889, P10321

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response