p53 antibody (Cy3)

Catalog No. ABIN723369

The Cy3-conjugated Mouse Monoclonal anti-p53 antibody (Clone D2F7) (ABIN723369) specifically detects p53 in WB. The antibody is reactive with Human and Rat samples.

Quick Overview for p53 antibody (Cy3) (ABIN723369)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is conjugated to Cy3

Application: Western Blotting (WB)

Clone: D2F7

Product Details anti-p53 Antibody (Cy3)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G.

Immunogen: KLH conjugated synthetic peptide derived from human P53(wt-p53)

Isotype: IgG

Application Details

Application Notes: IF(IHC-P)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Background:

Synonyms: Widespread p53, Wtp53, Antigen NY-CO-13, Cellular tumor antigen p53, Cys 51 Stop, HGNC11998, LFS1, p53, p53 Cellular Tumor Antigen, p53 Tumor Suppressor, Phosphoprotein p53, TP53, Transformation related protein 53, TRP53, Tumor protein p53, Tumour Protein p53, P53_HUMAN, TP53.

Background: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013].

Gene ID: 7157

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect