MEK1 antibody (C-Term) (HRP)

Catalog No. ABIN7565790

The HRP-conjugated Rabbit Polyclonal anti-MEK1 antibody (ABIN7565790) specifically detects MEK1 in WB, ELISA and FLISA. The antibody is reactive with Human samples.

Quick Overview for MEK1 antibody (C-Term) (HRP) (ABIN7565790)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, FLISA

Product Details anti-MEK1 Antibody (HRP)

Binding Specificity: C-Term

Purpose: MEK1 C-Term Antibody HRP

Cross-Reactivity (Details): This HRP conjugated affinity purified antibody is directed against human MEK1 protein.

Characteristics: Synonyms: rabbit anti-MEK1 antibody peroxidase conjugation, HRP conjugated rabbit anti-MEK1 antibody, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MAP2K1, MEK, MEK 1, MKK1, PRKMK1, MEK-1, ERK activator kinase 1, MAPK/ERK kinase 1

Purification: Anti-MEK1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-MEK 1 Peroxidase conjugated antibody has been tested by ELISA and is suitable for use in Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa. Western Blot Dilution: 1 μg/mL FLISA Dilution: 1:40,000 ELISA Dilution: 1:40,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1

Background: Background: MEK1 antibodies detect the MEK1 isoform. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling