MEK1 antibody (pSer222, pSer226)

Catalog No. ABIN7565795

The Mouse Monoclonal anti-MEK1 antibody is suitable to detect MEK1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for MEK1 antibody (pSer222, pSer226) (ABIN7565795)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 17C9-E1-H2-D7-H7-D5

Product Details anti-MEK1 Antibody

Binding Specificity: pSer222, pSer226

Purpose: MEK1 phosphoS222/MEK2 phosphoS226 Antibody

Cross-Reactivity (Details): This protein A purified mouse monoclonal antibody specifically binds to the human MEK1 pS222 and MEK2 pS226 phosphorylated sites.

Characteristics: Synonyms: mouse anti-MEK1 pS222/MEK2 pS226 Antibody, MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 Antibody, MAP2K2, MEK2, MKK2, PRKMK2, CFC4, MEK-2 Antibody, MEK1 and MEK2 antibody, phosphor MEK1/2

Purification: Anti-MEK1 pS222/MEK2 pS226 is purified from tissue culture supernatant by protein A purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-MEK1 pS222/MEK2 pS226 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues surrounding the S222-226 phosphorylation site conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 lambda

Application Details

Application Notes: Application Note: Anti-MEK1 pS222/MEK2 pS226 (MOUSE) antibody has been tested in ELISA and Western Blot. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa. Western Blot Dilution: User Optimized ELISA Dilution: 1:40,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1/MAP2K2

Background: Background: MEK1 pS222/MEK2 pS226 antibody detects MEK1 and MEK2. Mitogen-activated protein kinase kinase 1, (also known as MKK or MEK1), and Mitogen-activated protein kinase kinase 2, (also known as MEK2 or MKK2), are integral components of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 and 2 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling