PIAS1 antibody (Internal Region)

Catalog No. ABIN7565808

This anti-PIAS1 antibody is a Rabbit Polyclonal antibody detecting PIAS1 in WB, ELISA, DB and FM. Suitable for Human.

Quick Overview for PIAS1 antibody (Internal Region) (ABIN7565808)

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Fluorescence Microscopy (FM)

Product Details anti-PIAS1 Antibody

Binding Specificity: Internal Region

Purpose: PIAS1 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest reactivity to mouse 92.

Characteristics: Synonyms: Rabbit anti-PIAS1 Antibody, Protein Inhibitor Of Activated STAT 1, GBP, ZMIZ3, DDXBP1, DEAD/H (Asp-Glu-Ala-Asp/His) Box Binding Protein 1, GU/RH-II, E3 SUMO-protein ligase PIAS1, Gu-binding protein, GBP, AR Interacting Protein

Purification: Anti-PIAS1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-PIAS1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide from the internal region of human PIAS1.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-PIAS1 Antibody has been tested for use in ELISA, Western Blotting, and Dot Blotting. HEK293 WCL p/n (W09-000-365) and K-562 WCL p/n (W09-001-GJ7) were used as positive control lysates in western blot. Expect a band at approximately 72 kDa in Western Blots of specific cell lysates and tissues. Specific conditions for reactivity should be optimized by the end user. While not tested, it is likely Anti-PIAS1 antibody will work for immunofluorescence, IHC, and CHIP assays. Western Blot Dilution: 1:1000 ELISA Dilution: 5 μg/mL IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for PIAS1

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Alternative Name: PIAS1

Background: Background: The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated alpha-globin expression in erythroid cells. Anti-PIAS1 Antibody is useful for researchers interested in epigenetics and cancer research.

Gene ID: 8554

NCBI Accession: NP_057250

UniProt: O75925

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C