CRISPR-Cas9 (C-Term) antibody (Biotin)

Catalog No. ABIN7565920

This Rabbit Polyclonal antibody specifically detects CRISPR-Cas9 in WB, ELISA and FM. It exhibits reactivity toward Streptococcus pyogenes.

Quick Overview for CRISPR-Cas9 (C-Term) antibody (Biotin) (ABIN7565920)

Target: CRISPR-Cas9

Reactivity: Streptococcus pyogenes

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Product Details

Binding Specificity: C-Term

Purpose: Cas 9 Antibody Biotin Conjugated

Cross-Reactivity (Details): Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9.

Characteristics: Synonyms: rabbit anti-Cas9 antibody biotin conjugation, biotin conjugated rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Cas9 Biotin Conjugated Antibody has been tested for use in ELISA and suitable for Immunofluorescence and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:25,000-1:30,000 IF Microscopy Dilution: 1 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: CRISPR-Cas9

Alternative Name: cas9

Background: Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.

Gene ID: 901176

NCBI Accession: NP_269215

UniProt: Q99ZW2