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Leptin antibody (AA 101-154)

Name: Leptin antibody (AA 101-154)
SKU: ABIN725030
Price: 384.62 USD
Availability: InStock

The Rabbit Polyclonal anti-Leptin antibody has been validated for ELISA, IF (p), IF (cc), FACS, IHC (p) and IHC (fro). It is suitable to detect Leptin in samples from Mouse and Rat.

Formalin-fixed and paraffin embedded: rat liver labeled with Anti-Leptin Polyclonal Antibody (ABIN725030), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining (Leptin antibody (AA 101-154))

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Leptin antibody (AA 101-154))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse liver, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Leptin antibody (AA 101-154))

IHC (p)

3 images

Catalog No. ABIN725030

Quick Overview for Leptin antibody (AA 101-154) (ABIN725030)

Target

See all Leptin (LEP) Antibodies

Leptin (LEP)

Reactivity

Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Leptin antibody is un-conjugated

Application

ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Discover our top product LEP Primary Antibody

Binding Specificity
40

15

15

15

9

9

7

5

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 101-154

Cross-Reactivity

Mouse, Rat

Predicted Reactivity

Dog,Cow,Pig,Horse

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse Leptin

Isotype

IgG
anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 17-100) antibody
LEP Reactivity: Human, Mouse, Rat WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 8-37) antibody
LEP Reactivity: Human WB, IHC, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 29-160) antibody
LEP Reactivity: Cow WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 7-37), (N-Term) antibody
LEP Reactivity: Human WB, IF, FACS, EIA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Human WB Host: Mouse Monoclonal C5 unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Dog WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

Leptin (LEP)

Alternative Name

Leptin

Background

Synonyms: ob, obese, Leptin, Obesity factor, Lep
Background: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

Gene ID

16846

UniProt

P41160

Pathways

JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process