Leptin antibody (AA 101-154) (Cy3)

Catalog No. ABIN725033

The Rabbit Polyclonal anti-Leptin antibody has been validated for IF (p), IF (cc) and FACS. It is suitable to detect Leptin in samples from Mouse and Rat.

Quick Overview for Leptin antibody (AA 101-154) (Cy3) (ABIN725033)

Target: Leptin (LEP)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin antibody is conjugated to Cy3

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS)

Product Details anti-Leptin Antibody (Cy3)

Binding Specificity: AA 101-154

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Leptin

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background:

Synonyms: ob, obese, Leptin, Obesity factor, Lep

Background: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

Gene ID: 16846

UniProt: P41160

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process