SLC3A2 antibody (Extracellular)

Catalog No. ABIN7581980

The Rabbit Polyclonal anti-SLC3A2 antibody is suitable to detect SLC3A2 in samples from Mouse. It has been validated for WB, FACS, IHC and LCI.

Quick Overview for SLC3A2 antibody (Extracellular) (ABIN7581980)

Target: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC3A2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Product Details anti-SLC3A2 Antibody

Binding Specificity: AA 150-164, Extracellular

Purpose: A Rabbit Polyclonal antibody to CALHM2 (extracellular)

Sequence: (C)KGLVLGPIHK NQKDE

Specificity: Extracellular, C-terminus.

Predicted Reactivity: Rat - identical Human - 14 out of 15 amino acid residues identical

Characteristics: Anti-SLC3A2 (CD98) (extracellular) Antibody (ABIN7581980) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize SLC3A2 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KGLVLGPIHKNQKDE, corresponding to amino acid residues 150 - 164 of mouse SLC3A2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:400

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC3A2

Target: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Alternative Name: SLC3A2

Background: Solute Carrier Family 3 Member 2, 4F2 Cell-Surface Antigen Heavy Chain, 4F2HC, CD98 Antigen Heavy Chain, CD98HC,Solute carrier family 3 member 2 (SLC3A2), also known as 4F2 cell-surface antigen heavy chain (4F2HC) and CD98 antigen heavy chain (CD98hc), is a type II membrane protein belonging to the SLC3A/CD98 transporter family that plays a role in various physiological processes including amino acid transport, cell differentiation, migration, and proliferation.1,2,4SLC3A2 is a type II glycosylated protein composed of an extracellular domain, a transmembrane domain, and a cytoplasmic domain. SLC3A2 forms obligatory heterodimeric complexes with six members of the L-type amino acid transporters (LATs) SLC7A5-11, and SLC7A13.1The heteromeric complex between SLC3A2 and SLC7A5 (also known as LAT1) is one of the best characterized. SLC7A5 is responsible for the transport of large neutral amino acids, including leucine, across the cell membrane. SLC3A2 acts as a chaperone protein for SLC7A5, stabilizing its expression on the cell surface and assisting in its proper functioning. This partnership is essential for amino acid transport, cell growth, and protein synthesis. This complex is particularly important in cancer cells and immune cells, as it facilitates nutrient uptake and influences cell proliferation and immune responses.1-4In addition to SLC7A5, SLC3A2 also forms a complex with another SLC7 family member called SLC7A11 (also known as xCT). This complex is commonly referred to as the CD98/xCT or SLC3A2/SLC7A11.1-4xCT, is a cystine/glutamate antiporter that plays a crucial role in the cellular exchange of cystine (an amino acid involved in the synthesis of the antioxidant glutathione) for glutamate. This exchange is important for maintaining cellular redox balance and protecting cells from oxidative stress.1,3,5When SLC3A2 partners with SLC7A11, it facilitates the function of the xCT antiporter, allowing cells to import cystine while exporting glutamate. This process is essential for antioxidant defence and protecting cells from damage caused by reactive oxygen species (ROS). The SLC3A2/SLC7A11 complex has significant implications in cancer biology and the modulation of immune responses, as it can influence cellular redox status and affect processes related to cell proliferation and immune cell function.1,3,5

Gene ID: 17254

UniProt: P10852