GOT1 antibody

Catalog No. ABIN7595314

This anti-GOT1 antibody is a Guinea Pig Polyclonal antibody detecting GOT1 in WB, IHC and IHC (p). Suitable for Rat.

Quick Overview for GOT1 antibody (ABIN7595314)

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This GOT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GOT1 Antibody

Purpose: Guinea pig antibody to Aspartate Aminotransferase (AATC)

Specificity: Specific for AATC

Cross-Reactivity: Human, Mouse, Rat

Purification: Whole serum

Immunogen: A synthetic peptide from rat AATC conjugated to blue carrier protein was used as the antigen.

Application Details

Application Notes: IHC WB. A dilution of 1: 500 is recommended for IHC-P,WB. The optimal dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: Aspartate Aminotransferase

Background: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate regulates levels of mercaptopyruvate an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process