GFP antibody

Catalog No. ABIN7595339

This Carp Polyclonal antibody specifically detects GFP in WB, ELISA, IHC and FACS. It exhibits reactivity toward Aequorea victoria.

Quick Overview for GFP antibody (ABIN7595339)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Carp

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-GFP Antibody

Specificity: Specific for EGFP/GFP/Venus

Cross-Reactivity (Details): Any organism expressing EGFP/GFP/Venus.

Purification: IgM (mainly)

Immunogen: Recombinant EGFP/GFP/Venus protein was used as the antigen.

Isotype: IgM

Application Details

Application Notes: IHC WB ELISA FC. A working concentration of 5-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Use Rabbit anti Carp IgM (H+L) adaptobody at 1:1000 dilution to be able to use your usual anti rabbit secondary antibody.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term.

Expiry Date: 12 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: EGFP/GFP/Venus

Background: GFP is a naturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin's blue light blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer reporter of gene expression or as a measure of protein-protein interactions.