GFP antibody (AA 1-238) (FL490)

Catalog No. ABIN7595405

This Mouse Monoclonal antibody specifically detects GFP in IHC and ICC. It exhibits reactivity toward Jellyfish.

Quick Overview for GFP antibody (AA 1-238) (FL490) (ABIN7595405)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Jellyfish

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to FL490

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: N86-38

Product Details anti-GFP Antibody (FL490)

Binding Specificity: AA 1-238

Purpose: Anti-GFP Antibody FL490 Conjugate

Specificity: Recognizes Cyan and Yellow fluorescent protein (CFP and YFP, respectively)No off-targets reported for Red fluorescent protein (dsRed)

Characteristics:

Description: Our Anti-GFP mouse monoclonal primary antibody is produced in-house from hybridoma clone N86/38. It detects GFP, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Manufacturer Comment: We produce our GFP mouse monoclonal primary antibody from hybridoma clone N86/38. It is great in IHC, ICC and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb.

Purity: > 90 % specific antibody

Immunogen: Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 12 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background:

Synonyms: Green fluorescent protein

Target Description: Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Gene Name Alternatives: GFP

Molecular Weight: 27 kDa