GFP antibody (AA 1-238)

Catalog No. ABIN7595930

This Mouse Monoclonal antibody specifically detects GFP in WB, ELISA, IHC, IP, ICC and ChIP. It exhibits reactivity toward Jellyfish.

Quick Overview for GFP antibody (AA 1-238) (ABIN7595930)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Jellyfish

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Clone: N86-8

Product Details anti-GFP Antibody

Binding Specificity: AA 1-238

Purpose: Anti-GFP Antibody

Specificity: Recognizes Cyan and Yellow fluorescent protein (CFP and YFP, respectively)No off-targets reported for Red fluorescent protein (dsRed)

Characteristics: Our Anti-GFP mouse monoclonal primary antibody is produced in-house from hybridoma clone N86/8. It detects n/a GFP, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA, ChIP.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Purity: Purified mAbs are >90 % specific antibody.

Immunogen: Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Isotype: IgG2a

Application Details

Application Notes:

Dilution Range: ICC: 1:500

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background:

Synonyms: Green fluorescent protein

Description Logic: Our Anti-GFP mouse monoclonal primary antibody is produced from hybridoma clone N86/8. It detects n/a GFP, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA, ChIP.

Gene Name Alternatives: GFP

Molecular Weight: 27 kDa

UniProt: P42212