RAD17 antibody (AA 1-267)

Catalog No. ABIN7599188

The Rabbit Polyclonal anti-RAD17 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect RAD17 in samples from Mouse and Rat.

Quick Overview for RAD17 antibody (AA 1-267) (ABIN7599188)

Target: RAD17

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-RAD17 Antibody

Binding Specificity: AA 1-267

Purpose: Anti-Rad17 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Rad17 Antibody Picoband® (ABIN7599188). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Rad17 recombinant protein (Position: M1-Q267).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Bao, S., Shen, X., Shen, K., Liu, Y., Wang, X.-F. The mammalian Rad24 homologous to yeast Saccharomyces cerevisiae Rad24 and Schizosaccharomyces pombe Rad17 is involved in DNA damage checkpoint. Cell Growth Diff. 9: 961-967, 1998. 2. Bao, S., Tibbetts, R. S., Brumbaugh, K. M., Fang, Y., Richardson, D. A., Ali, A., Chen, S. M., Abraham, R. T., Wang, X.-F. ATR/ATM-mediated phosphorylation of human Rad17 is required for genotoxic stress responses. Nature 411: 969-974, 2001. 3. Bluyssen, H. A. R., Naus, N. C., van Os, R. I., Jaspers, I., Hoeijmakers, J. H. J., de Klein, A. Human and mouse homologues of the Schizosaccharomyces pombe rad17+ cell cycle checkpoint control gene. Genomics 55: 219-228, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAD17

Target: RAD17

Alternative Name: Rad17

Background:

Synonyms: T-cell surface glycoprotein CD1b, CD1b, CD1B

Tissue Specificity: Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.

Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Molecular Weight: 77 kDa

Gene ID: 19356