CPT1A antibody (AA 122-556)

Catalog No. ABIN7599853

This anti-CPT1A antibody is a Rabbit Polyclonal antibody detecting CPT1A in WB, IHC, ELISA, ICC, IF and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for CPT1A antibody (AA 122-556) (ABIN7599853)

Target: CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPT1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-CPT1A Antibody

Binding Specificity: AA 122-556

Purpose: Anti-CPT1A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CPT1A Antibody Picoband® (ABIN7599853). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CPT1A recombinant protein (Position: M122-K556).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Andersen, M. K., Hansen, T. Genetics of metabolic traits in Greenlanders: lessons from an isolated population. J. Intern. Med. 284: 464-477, 2018. 2. Andersen, M. K., Jorsboe, E., Sandholt, C. H., Grarup, N., Jorgensen, M. E., Faergeman, N. J., Bjerregaard, P., Pedersen, O., Moltke, I., Hansen, T., Albrechtsen, A. Identification of novel genetic determinants of erythrocyte membrane fatty acid composition among Greenlanders. PLoS Genet. 12: e1006119, 2016. Note: Electronic Article. 3. Bergstrom, J. P., Reitz, R. C. Studies on carnitine palmitoyl transferase: the similar nature of CPTi (inner form) and CPTo (outer form). Arch. Biochem. Biophys. 204: 71-78, 1980.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CPT1A

Target: CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))

Alternative Name: CPT1A

Background:

Synonyms: CD59A glycoprotein, MAC-inhibitory protein, MAC-IP, Membrane attack complex inhibition factor, MACIF, Protectin, CD59, Cd59a, Cd59

Tissue Specificity: Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.

Background: The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 88 kDa

Gene ID: 1374

UniProt: P50416

Pathways: AMPK Signaling, Regulation of Lipid Metabolism by PPARalpha, Feeding Behaviour, Monocarboxylic Acid Catabolic Process