Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

TDG antibody (AA 15-370)

The Rabbit Polyclonal anti-TDG antibody has been validated for WB, ELISA and FACS. It is suitable to detect TDG in samples from Human and Monkey.
Catalog No. ABIN7600107

Quick Overview for TDG antibody (AA 15-370) (ABIN7600107)

Target

See all TDG Antibodies
TDG (Thymine-DNA Glycosylase (TDG))

Reactivity

  • 50
  • 19
  • 11
  • 2
  • 1
Human, Monkey

Host

  • 38
  • 12
Rabbit

Clonality

  • 41
  • 10
Polyclonal

Conjugate

  • 44
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This TDG antibody is un-conjugated

Application

  • 40
  • 13
  • 11
  • 11
  • 6
  • 6
  • 4
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB), ELISA, Flow Cytometry (FACS)
  • Binding Specificity

    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 15-370

    Purpose

    Anti-TDG Antibody Picoband®

    Cross-Reactivity (Details)

    No cross-reactivity with other proteins.

    Characteristics

    Anti-TDG Antibody Picoband® (ABIN7600107). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Purification

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human TDG recombinant protein (Position: Q15-R370).

    Isotype

    IgG
  • Application Notes

    Western blot, 0.25-0.5 μg/mL, Human, Monkey
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Baba, D., Maita, N., Jee, J.-G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H., Shirakawa, M. Crystal structure of thymine DNA glycosylase conjugated to SUMO-1. (Letter) Nature 435: 979-982, 2005. 2. Barrett, T. E., Savva, R., Panayotou, G., Barlow, T., Brown, T., Jiricny, J., Pearl, L. H. Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions. Cell 92: 117-129, 1998. 3. Cortazar, D., Kunz, C., Selfridge, J., Lettieri, T., Saito, Y., MacDougall, E., Wirz, A., Schuermann, D., Jacobs, A. L., Siegrist, F., Steinacher, R., Jiricny, J., Bird, A., Schar, P. Embryonic lethal phenotype reveals a function of TDG in maintaining epigenetic stability. Nature 470: 419-423, 2011.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    Storage

    4 °C,-20 °C

    Storage Comment

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • Target

    TDG (Thymine-DNA Glycosylase (TDG))

    Alternative Name

    TDG

    Background

    Synonyms: Kelch repeat and BTB domain-containing protein 2, BTB and kelch domain-containing protein 1, KBTBD2, BKLHD1, KIAA1489

    Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

    Background: G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

    Molecular Weight

    46 kDa

    Gene ID

    6996

    UniProt

    Q13569

    Pathways

    DNA Damage Repair, Chromatin Binding
You are here:
Chat with us!