Choline Acetyltransferase antibody (AA 19-612)

Catalog No. ABIN7600421

This anti-Choline Acetyltransferase antibody is a Rabbit Polyclonal antibody detecting Choline Acetyltransferase in WB, IHC and ELISA. Suitable for Mouse and Rat.

Quick Overview for Choline Acetyltransferase antibody (AA 19-612) (ABIN7600421)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Choline Acetyltransferase Antibody

Binding Specificity: AA 19-612

Purpose: Anti-Choline Acetyltransferase/Chat Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Choline Acetyltransferase/Chat Antibody (ABIN7600421). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat Choline Acetyltransferase/Chat recombinant protein (Position: E19-D612).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: Chat

Background:

Synonyms: Choline O-acetyltransferase (EC:2.3.1.6); CHOACTase; ChAT; Choline acetylase; Chat

Background: Choline acetyltransferase  (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.

Molecular Weight: 71 kDa

Gene ID: 290567

UniProt: P32738

Pathways: Skeletal Muscle Fiber Development