CD25 antibody (AA 22-268)

Catalog No. ABIN7600684

The Rabbit Polyclonal anti-CD25 antibody is suitable to detect CD25 in samples from Mouse and Rat. It has been validated for IHC, ELISA, IF and ICC.

Quick Overview for CD25 antibody (AA 22-268) (ABIN7600684)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CD25 Antibody

Binding Specificity: AA 22-268

Purpose: Anti-Il2ra Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Il2ra Antibody (ABIN7600684). Tested in ELISA, IF, IHC, ICC applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Il2ra recombinant protein (Position: E22-I268).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: Il2ra

Background:

Synonyms: GTP-binding nuclear protein Ran,Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein,RAN,ARA24,OK/SW-cl.81,

Tissue Specificity: Expressed in a variety of tissues. .

Background: IL2RA(Interleukin 2 receptor, alpha), also called IL2R, IL2R, Alpha Chain or CD25, is a protein that in humans is encoded by the IL2RA gene.The IL2RA gene is mapped on 10p15.1. IL2RA is composed of strand-swapped "sushi-like" domains, unlike the classical cytokine receptor fold. As a result of this domain swap, IL2RA uses a composite surface to dock into a groove on IL2 that also serves as a binding site for antagonist drugs.Homodimeric alpha chains(IL2RA) result in low-affinity receptor, while homodimeric beta(IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Infection by the protozoan Trypanosoma cruzi causes Chagas disease, characterized by a reduction in the amount of IL2RA expressed on the surface of immune cells. This leads to chronic immune suppression, becoming increasingly severe over the course of many years and ultimately resulting in death if left untreated.

Molecular Weight: 50 kDa

Gene ID: 16184

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation