AGT antibody (AA 273-458)

Catalog No. ABIN7601044

The Rabbit Polyclonal anti-AGT antibody is suitable to detect AGT in samples from Human. It has been validated for WB, IHC, ELISA and FACS.

Quick Overview for AGT antibody (AA 273-458) (ABIN7601044)

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-AGT Antibody

Binding Specificity: AA 273-458

Purpose: Anti-Angiotensinogen/AGT Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Angiotensinogen/AGT Antibody (ABIN7601044). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Angiotensinogen/AGT recombinant protein (Position: D273-R458).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for AGT

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Alternative Name: AGT

Background:

Synonyms: Angiotensinogen,Serpin A8,Angiotensin-1,Angiotensin 1-10,Angiotensin I,Ang I,Angiotensin-2,Angiotensin 1-8,Angiotensin II,Ang II,Angiotensin-3,Angiotensin 2-8,Angiotensin III,Ang III,Des-Asp[1]-angiotensin II,Angiotensin-4,Angiotensin 3-8,Angiotensin IV,Ang IV,Angiotensin 1-9,Angiotensin 1-7,Angiotensin 1-5,Angiotensin 1-4,Agt,Serpina8,

Tissue Specificity: Expressed by the liver and secreted in plasma.

Background: AGT(ANGIOTENSINOGEN), also called SERPINA8, is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Molecular Weight: 53 kDa

Gene ID: 183

UniProt: P01019

Pathways: JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity