EPH Receptor B2 antibody (AA 278-540)

Catalog No. ABIN7601057

The Rabbit Polyclonal anti-EPH Receptor B2 antibody (ABIN7601057) specifically detects EPH Receptor B2 in WB, ELISA, FACS, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EPH Receptor B2 antibody (AA 278-540) (ABIN7601057)

Target: EPH Receptor B2 (EPHB2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-EPH Receptor B2 Antibody

Binding Specificity: AA 278-540

Purpose: Anti-Eph receptor B2/EPHB2 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Eph receptor B2/EPHB2 Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Eph receptor B2/EPHB2 recombinant protein (Position: K278-K540).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EPHB2

Background:

Background: Ephrin type-B receptor 2 is a protein that in humans is encoded by the EPHB2 gene. This gene is mapped to 1p36.12. This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in biional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple transcript variants.

Gene Full Name: EPH receptor B2

Molecular Weight: 117 kDa

Gene ID: 2048

UniProt: P29323

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins