Poliovirus Receptor antibody (AA 28-331)

Catalog No. ABIN7601071

The Mouse Monoclonal anti-Poliovirus Receptor antibody has been validated for WB, IHC and FACS. It is suitable to detect Poliovirus Receptor in samples from Human and Mouse.

Quick Overview for Poliovirus Receptor antibody (AA 28-331) (ABIN7601071)

Target: Poliovirus Receptor (PVR)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Poliovirus Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 5I13D1

Product Details anti-Poliovirus Receptor Antibody

Binding Specificity: AA 28-331

Purpose: Anti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 5I13D1)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 5I13D1) (ABIN7601071). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Poliovirus Receptor/PVR recombinant protein (Position: D28-E331).

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Bottino, C., Castriconi, R., Pende, D., Rivera, P., Nanni, M., Carnemolla, B., Cantoni, C., Grassi, J., Marcenaro, S., Reymond, N., Vitale, M., Moretta, L., Lopez, M., Moretta, A. Identification of PVR (CD155) and nectin-2 (CD112) as cell surface ligands for the human DNAM-1 (CD226) activating molecule. J. Exp. Med. 198: 557-567, 2003. 2. Fuchs, A., Cella, M., Giurisato, E., Shaw, A. S., Colonna, M. Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155). J. Immun. 172: 3994-3998, 2004. 3. Gromeier, M., Solecki, D., Patel, D. D., Wimmer, E. Expression of the human poliovirus receptor/CD155 gene during development of the central nervous system: implications for the pathogenesis of poliomyelitis. Virology 273: 248-257, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Poliovirus Receptor

Target: Poliovirus Receptor (PVR)

Alternative Name: PVR

Background:

Synonyms: Major vault protein, MVP, Lung resistance-related protein, MVP, LRP

Tissue Specificity: Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.

Background: CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that in humans is encoded by the PVR gene. The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 70-80 kDa

Gene ID: 5817

UniProt: P15151

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome