RNF25 antibody (AA 44-344)

Catalog No. ABIN7601741

The Rabbit Polyclonal anti-RNF25 antibody (ABIN7601741) specifically detects RNF25 in WB, IHC, ELISA, FACS and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RNF25 antibody (AA 44-344) (ABIN7601741)

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-RNF25 Antibody

Binding Specificity: AA 44-344

Purpose: Anti-RNF25 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human RNF25 shares 83.7% amino acid (aa) sequence identity with mouse RNF25.

Characteristics: Anti-RNF25 Antibody. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RNF25 recombinant protein (Position: E44-R344). Human RNF25 shares 83.7% amino acid (aa) sequence identity with mouse RNF25.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RNF25

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Alternative Name: RNF25

Background:

Background: E3 ubiquitin-protein ligase RNF25 is an enzyme that in humans is encoded by the RNF25 gene. The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.

Gene Full Name: ring finger protein 25

Molecular Weight: 62 kDa

Gene ID: 64320

UniProt: Q96BH1