POLA1 antibody (AA 45-1429)

Catalog No. ABIN7601759

This anti-POLA1 antibody is a Rabbit Polyclonal antibody detecting POLA1 in WB, ELISA, ICC, IF and FACS. Suitable for Human.

Quick Overview for POLA1 antibody (AA 45-1429) (ABIN7601759)

Target: POLA1 (DNA Polymerase alpha (POLA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-POLA1 Antibody

Binding Specificity: AA 45-1429

Purpose: Anti-POLA1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-POLA1 Antibody Picoband® (ABIN7601759). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLA1 recombinant protein (Position: E45-D1429).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Adler, D. A., Tseng, B. Y., Wang, T. S.-F., Disteche, C. M. Physical mapping of the genes for three components of the mouse DNA replication complex: polymerase alpha to the X chromosome, primase p49 subunit to chromosome 10, and primase p58 subunit to chromosome 1. Genomics 9: 642-646, 1991. 2. Hanaoka, F., Tandai, M., Miyazawa, H., Murakami, Y., Hori, T., Yamada, M. Assignment of human DNA polymerase alpha gene (POLA) to the X chromosome. (Abstract) Cytogenet. Cell Genet. 40: 647 only, 1985. 3. Miyazawa, H., Tandai, M., Hanaoka, F., Yamada, M., Hori, T., Shimizu, K., Sekiguchi, M. Identification of a DNA segment containing the human DNA polymerase alpha gene. Biochem. Biophys. Res. Commun. 139: 637-643, 1986.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POLA1

Target: POLA1 (DNA Polymerase alpha (POLA1))

Alternative Name: POLA1

Background:

Synonyms: Tumor necrosis factor ligand superfamily member 18, Activation-inducible TNF-related ligand, AITRL, Glucocorticoid-induced TNF-related ligand, hGITRL, TNFSF18, AITRL, GITRL, TL6, UNQ149/PRO175,

Tissue Specificity: Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.

Background: DNA polymerase alpha catalytic subunit is an enzyme that in humans is encoded by the POLA1 gene. This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication.

Molecular Weight: 180 kDa

Gene ID: 5422

UniProt: P09884

Pathways: SARS-CoV-2 Protein Interactome