MUS81 antibody (AA 6-545)

Catalog No. ABIN7602139

The Rabbit Polyclonal anti-MUS81 antibody has been validated for ELISA, WB and FACS. It is suitable to detect MUS81 in samples from Human and Rat.

Quick Overview for MUS81 antibody (AA 6-545) (ABIN7602139)

Target: MUS81 (MUS81 Endonuclease Homolog (MUS81))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUS81 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-MUS81 Antibody

Binding Specificity: AA 6-545

Purpose: Anti-MUS81 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MUS81 Antibody Picoband® (ABIN7602139). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MUS81 recombinant protein (Position: R6-C545).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, X.-B., Melchionna, R., Denis, C.-M., Gaillard, P.-H. L., Blasina, A., Van de Weyer, I., Boddy, M. N., Russell, P., Vialard, J., McGowan, C. H. Human Mus81-associated endonuclease cleaves Holliday junctions in vitro. Molec. Cell 8: 1117-1127, 2001. 2. Ciccia, A., Constantinou, A., West, S. C. Identification and characterization of the human Mus81-Eme1 endonuclease. J. Biol. Chem. 278: 25172-25178, 2003. 3. Ciccia, A., Ling, C., Coulthard, R., Yan, Z., Xue, Y., Meetei, A. R., Laghmani, E. H., Joenje, H., McDonald, N., de Winter, J. P., Wang, W., West, S. C. Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM. Molec. Cell 25: 331-343, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MUS81

Target: MUS81 (MUS81 Endonuclease Homolog (MUS81))

Alternative Name: MUS81

Background:

Synonyms: Lipopolysaccharide-binding protein, LBP, Lbp,

Tissue Specificity: Detected in blood serum (at protein level).

Background: Crossover junction endonuclease MUS81 is an enzyme that in humans is encoded by the MUS81 gene. This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks.

Molecular Weight: 70 kDa

Gene ID: 80198

Pathways: DNA Damage Repair