Acetyl-CoA Carboxylase beta antibody (C-Term)

Catalog No. ABIN7602757

The Rabbit Polyclonal anti-Acetyl-CoA Carboxylase beta antibody has been validated for WB, IHC and FACS. It is suitable to detect Acetyl-CoA Carboxylase beta in samples from Human, Mouse and Rat.

Quick Overview for Acetyl-CoA Carboxylase beta antibody (C-Term) (ABIN7602757)

Target: Acetyl-CoA Carboxylase beta (ACACB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetyl-CoA Carboxylase beta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Acetyl-CoA Carboxylase beta Antibody

Binding Specificity: C-Term

Purpose: Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody (ABIN7602757). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Acetyl Coenzyme A Carboxylase/ACACB, which shares 64.7% amino acid (aa) sequence identity with mouse Acetyl Coenzyme A Carboxylase/ACACB.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Acetyl-CoA Carboxylase beta

Target: Acetyl-CoA Carboxylase beta (ACACB)

Alternative Name: ACACB

Background:

Synonyms: Acetyl-CoA carboxylase 2, ACC-beta, Biotin carboxylase, ACACB, ACC2, ACCB

Tissue Specificity: Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon (PubMed:9099716). Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level) (PubMed:19190759). Isoform 3 is detected at high levels in adipose tissue with lower levels in heart, liver, skeletal muscle and testis (PubMed:19190759).

Background: Acetyl-CoA carboxylase 2 also known as ACC-beta or ACC2 is an enzyme that in humans is encoded by the ACACB gene. It is mapped to 12q24.11. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms.

Molecular Weight: 277 kDa

Gene ID: 32

UniProt: O00763

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process