eIF4EBP1 antibody (C-Term)

Catalog No. ABIN7602881

This anti-eIF4EBP1 antibody is a Rabbit Polyclonal antibody detecting eIF4EBP1 in WB, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for eIF4EBP1 antibody (C-Term) (ABIN7602881)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eIF4EBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-eIF4EBP1 Antibody

Binding Specificity: C-Term

Purpose: Anti-EIF4EBP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EIF4EBP1 Antibody Picoband® (ABIN7602881). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human EIF4EBP1, which shares 75% and 81.2% amino acid (aa) sequence identity with mouse and rat EIF4EBP1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Colina, R., Costa-Mattioli, M., Dowling, R. J. O., Jaramillo, M., Tai, L.-H., Breitbach, C. J., Martineau, Y., Larsson, O., Rong, L., Svitkin, Y. V., Makrigiannis, A. P., Bell, J. C., Sonenberg, N. Translational control of the innate immune response through IRF-7. Nature 452: 323-328, 2008. 2. Demontis, F., Perrimon, N. FOXO/4E-BP signaling in Drosophila muscles regulates organism-wide proteostasis during aging. Cell 143: 813-825, 2010. 3. Dowling, R. J. O., Topisirovic, I., Alain, T., Bidinosti, M., Fonseca, B. D., Petroulakis, E., Wang, X., Larsson, O., Selvaraj, A., Liu, Y., Kozma, S. C., Thomas, G., Sonenberg, N. mTORC1-mediated cell proliferation, but not cell growth, controlled by the 4E-BPs. Science 328: 1172-1176, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: EIF4EBP1

Background:

Synonyms: Large proline-rich protein BAG6, BAG family molecular chaperone regulator 6, BCL2-associated athanogene 6, BAG-6, HLA-B-associated transcript 3, Protein G3, Protein Scythe, BAG6, BAT3

Tissue Specificity: Expressed by immature dendritic cells.

Background: Eukaryotic translation initiation factor 4E-binding protein 1 (also known as 4E-BP1) is a protein that in humans is encoded by the EIF4EBP1 gene. This gene encodes one member of a family of translation repressor proteins. The protein ly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Molecular Weight: 17 kDa

Gene ID: 1978

UniProt: Q13541

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling