JAK2 antibody (Cytosolic)

Catalog No. ABIN7602956

The Rabbit Monoclonal anti-JAK2 antibody (Clone COG-10) (ABIN7602956) specifically detects JAK2 in WB, IHC, ICC, IF and IP. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for JAK2 antibody (Cytosolic) (ABIN7602956)

Target: JAK2 (Janus Kinase 2 (JAK2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This JAK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: COG-10

Product Details anti-JAK2 Antibody

Binding Specificity: Cytosolic

Purpose: Anti-JAK2 Monoclonal Antibody

Characteristics: Anti-JAK2 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2.

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000IHC 1:50-200ICC/IF 1:50-200IP 1:50-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Alternative Name: JAK2

Background: Janus kinase 2

Molecular Weight: 131 kDa

Gene ID: 3717

UniProt: O60674

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response