INSIG1 antibody (N-Term)

Catalog No. ABIN7603176

This Rabbit Polyclonal antibody specifically detects INSIG1 in WB and FACS. It exhibits reactivity toward Mouse and Rat.

Quick Overview for INSIG1 antibody (N-Term) (ABIN7603176)

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INSIG1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-INSIG1 Antibody

Binding Specificity: N-Term

Purpose: Anti-Insig1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Insig1 Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of rat Insig1, which shares 71.4% and 92.9% amino acid (aa) sequence identity with human and mouse Insig1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for INSIG1

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Alternative Name: Insig1

Background:

Background: Insulin induced gene 1, also known as INSIG1, is a protein which in humans is encoded by the INSIG1 gene. This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants.

Gene Full Name: insulin induced gene 1

Molecular Weight: 30 kDa

Gene ID: 64194

UniProt: Q08755

Pathways: ER-Nucleus Signaling