PUMA antibody (N-Term)

Catalog No. ABIN7603233

This Rabbit Polyclonal antibody specifically detects PUMA in WB. It exhibits reactivity toward Human.

Quick Overview for PUMA antibody (N-Term) (ABIN7603233)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PUMA Antibody

Binding Specificity: N-Term

Purpose: Anti-PUMA/BBC3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PUMA/BBC3 Antibody Picoband® (ABIN7603233). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PUMA/BBC3, which shares 95.7% amino acid (aa) sequence identity with mouse and rat BBC3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human
1. Nakano K, Vousden KH (March 2001). "PUMA, a novel proapoptotic gene, is induced by p53". Mol. Cell 7 (3): 683-94. 2. Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T (September 2001). "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals". Proc. Natl. Acad. Sci. U.S.A. 98 (20): 11318-23. 3. Yu, J., Zhang, L., Hwang, P. M., Kinzler, K. W., Vogelstein, B. : PUMA induces the rapid apoptosis of colorectal cancer cells. Molec. Cell 7: 673-682, 2001. 4. Nakano, K., Vousden, K. H. : PUMA, a novel proapoptotic gene, is induced by p53. Molec. Cell 7: 683-694, 2001. 5. Chipuk, J. E., Bouchier-Hayes, L., Kuwana, T., Newmeyer, D. D., Green, D. R. : PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. Science 309: 1732-1735, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: BBC3

Background:

Synonyms: Smoothelin, SMTN, SMSMO

Tissue Specificity: Smooth muscle, contractile or vascular (for the long form).

Background: The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91 % amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.

Molecular Weight: 18 kDa

Gene ID: 27113

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity