TLR8 antibody (N-Term)

Catalog No. ABIN7603240

The Rabbit Polyclonal anti-TLR8 antibody has been validated for WB and IHC. It is suitable to detect TLR8 in samples from Mouse and Rat.

Quick Overview for TLR8 antibody (N-Term) (ABIN7603240)

Target: TLR8 (Toll-Like Receptor 8 (TLR8))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TLR8 Antibody

Binding Specificity: N-Term

Purpose: Anti-Tlr8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Tlr8 Antibody Picoband® (ABIN7603240). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse Tlr8, which shares 76.5% amino acid (aa) sequence identity with human TLR8.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
1. Chuang, T.-H., Ulevitch, R. J.Cloning and characterization of a sub-family of human Toll-like receptors: hTLR7, hTLR8 and hTLR9.Europ. Cytokine Netw. 11: 372-378, 2000. 2. Kadowaki, N., Ho, S., Antonenko, S., de Waal Malefyt, R., Kastelein, R. A., Bazan, F., Liu, Y.-J.Subsets of human dendritic cell precursors express different Toll-like receptors and respond to different microbial antigens.J. Exp. Med. 194: 863-869, 2001. 3. Peng, G., Guo, Z., Kiniwa, Y., Voo, K., Peng, W., Fu, T., Wang, D. Y., Li, Y., Wang, H. Y., Wang, R.-F.Toll-like receptor 8-mediated reversal of CD4+ regulatory T cell function.Science 309: 1380-1384, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TLR8

Target: TLR8 (Toll-Like Receptor 8 (TLR8))

Alternative Name: Tlr8

Background:

Synonyms: Nuclear factor 1 B-type, NF1-B, Nuclear factor 1/B, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/B, NF-I/B, NFI-B, TGGCA-binding protein, NFIB

Background: TLR8 (Toll-like receptor 8) is a protein that in humans is encoded by the TLR8 gene. TLR8 has also been designated as CD288 (cluster of differentiation 288).The TLR8 gene is mapped to Xp22.3-p22.2 by Chuang and Ulevitch (2000) and Du et al. (2000). The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. TLR8 recognises G-rich oligonucleotides.

Molecular Weight: 119 kDa

Gene ID: 170744

UniProt: P58682

Pathways: TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades