H2AFX antibody (pSer139)

Catalog No. ABIN7603274

This anti-H2AFX antibody is a Rabbit Monoclonal antibody detecting H2AFX in WB, IHC, IF, ICC and IP. Suitable for Human, Mouse and Rat.

Quick Overview for H2AFX antibody (pSer139) (ABIN7603274)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: AbH41

Product Details anti-H2AFX Antibody

Binding Specificity: pSer139

Purpose: Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody

Characteristics: Anti-Phospho-Histone H2A.X H2AFX Monoclonal Antibody (ABIN7603274). Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-Histone H2A.X (S139)

Isotype: IgG

Application Details

Application Notes: WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AFX

Background:

Synonyms: Red fluorescent protein eqFP611,GFP-like chromoprotein,

Tissue Specificity: Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .

Molecular Weight: 15 kDa

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus