ERK1 antibody (pThr185, pThr202)

Catalog No. ABIN7603387

The Rabbit Monoclonal anti-ERK1 antibody has been validated for WB, IHC, IF, IP and ICC. It is suitable to detect ERK1 in samples from Human, Mouse and Rat.

Quick Overview for ERK1 antibody (pThr185, pThr202) (ABIN7603387)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 18M06

Product Details anti-ERK1 Antibody

Binding Specificity: pThr185, pThr202

Purpose: Anti-Phospho-ERK1 (T202) + ERK2 (T185) Rabbit Monoclonal Antibody

Characteristics: Anti-Phospho-ERK1 + ERK2 Rabbit Monoclonal Antibody (ABIN7603387). Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-ERK1 (T202) + ERK2 (T185)

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3

Background:

Synonyms: Mitogen-activated protein kinase 3,MAP kinase 3,MAPK 3,2.7.11.24,ERT2,Extracellular signal-regulated kinase 1,ERK-1,Insulin-stimulated MAP2 kinase,MAP kinase isoform p44,p44-MAPK,Microtubule-associated protein 2 kinase,p44-ERK1,MAPK3,ERK1, PRKM3,

Tissue Specificity: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.

Molecular Weight: 50-70 kDa

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins