ERK1 antibody

Catalog No. ABIN7604776

The Rabbit Monoclonal anti-ERK1 antibody has been validated for WB, IHC, IF, IP, ICC and FACS. It is suitable to detect ERK1 in samples from Human and Mouse.

Quick Overview for ERK1 antibody (ABIN7604776)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: ADF-13

Product Details anti-ERK1 Antibody

Purpose: Anti-ERK1 MAPK3 Rabbit Monoclonal Antibody

Characteristics: Anti-ERK1 MAPK3 Rabbit Monoclonal Antibody (ABIN7604776). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human ERK1

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:250
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3

Background:

Synonyms: Mitogen-activated protein kinase 3,MAP kinase 3,MAPK 3,2.7.11.24,ERT2,Extracellular signal-regulated kinase 1,ERK-1,Insulin-stimulated MAP2 kinase,MAP kinase isoform p44,p44-MAPK,Microtubule-associated protein 2 kinase,p44-ERK1,MAPK3,ERK1, PRKM3,

Tissue Specificity: Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. .

Molecular Weight: 31 kDa, 28 kDa, 17 kDa

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins