Glutaminase antibody

Catalog No. ABIN7604990

This anti-Glutaminase antibody is a Rabbit Monoclonal antibody detecting Glutaminase in WB, IHC, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for Glutaminase antibody (ABIN7604990)

Target: Glutaminase (GLS)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: FIA-7

Product Details anti-Glutaminase Antibody

Purpose: Anti-Glutaminase GLS Rabbit Monoclonal Antibody

Characteristics: Anti-Glutaminase GLS Rabbit Monoclonal Antibody (ABIN7604990). Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Glutaminase

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: GLS

Background:

Synonyms: Glutaminase kidney isoform, mitochondrial,GLS,3.5.1.2,K-glutaminase,L-glutamine amidohydrolase,GLS,GLS1, KIAA0838,

Tissue Specificity: Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle. .

Molecular Weight: 70 kDa

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect