IL-1 beta antibody

Catalog No. ABIN7605241

The Rabbit Monoclonal anti-IL-1 beta antibody is suitable to detect IL-1 beta in samples from Mouse and Rat. It has been validated for WB, IP and FACS.

Quick Overview for IL-1 beta antibody (ABIN7605241)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 28I10

Product Details anti-IL-1 beta Antibody

Purpose: Anti-IL-1 beta Rabbit Monoclonal Antibody

Characteristics: Anti-IL-1 beta Rabbit Monoclonal Antibody (ABIN7605241). Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Mouse.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human IL-1 beta

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: Il1b

Background:

Synonyms: Interleukin-1 beta,IL-1 beta,Catabolin,IL1B,IL1F2,

Tissue Specificity: Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. .

Molecular Weight: 31 kDa, 28 kDa, 17 kDa

UniProt: P10749

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome