EGLN1 antibody

Catalog No. ABIN7605935

The Rabbit Monoclonal anti-EGLN1 antibody (Clone ACEF-5) (ABIN7605935) specifically detects EGLN1 in WB and IP. The antibody is reactive with Human samples.

Quick Overview for EGLN1 antibody (ABIN7605935)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EGLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: ACEF-5

Product Details anti-EGLN1 Antibody

Purpose: Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody

Characteristics: Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody. Tested in WB, IP applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PHD2 / prolyl hydroxylase Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000IP 1:20

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: EGLN1

Background: Egl-9 family hypoxia inducible factor 1

Molecular Weight: 52 kDa

Gene ID: 54583

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect