PYCARD antibody

Catalog No. ABIN7606534

The Rabbit Monoclonal anti-PYCARD antibody is suitable to detect PYCARD in samples from Human. It has been validated for WB, IF, FACS and ICC.

Quick Overview for PYCARD antibody (ABIN7606534)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: FFB-16

Product Details anti-PYCARD Antibody

Purpose: Anti-TMS1 PYCARD Rabbit Monoclonal Antibody

Characteristics: Anti-TMS1 PYCARD Rabbit Monoclonal Antibody (ABIN7606534). Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human TMS1

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
ICC/IF 1:50-200
FC 1:20

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: PYCARD

Background:

Synonyms: Apoptosis-associated speck-like protein containing a CARD,hASC,Caspase recruitment domain-containing protein 5,PYD and CARD domain-containing protein,Target of methylation-induced silencing 1,PYCARD,ASC, CARD5, TMS1,

Tissue Specificity: Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells.

Molecular Weight: 53 kDa

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome