IL-1 beta antibody (AA 101-200)

Catalog No. ABIN728503

This Rabbit Polyclonal antibody specifically detects IL-1 beta in WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) and DB. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Cow and Pig and has been mentioned in 16+ publications.

Quick Overview for IL-1 beta antibody (AA 101-200) (ABIN728503)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Product Details anti-IL-1 beta Antibody

Binding Specificity: AA 101-200

Specificity: Predicted to work with Mouse and Rat samples, but with lower affinity.

Cross-Reactivity: Cow, Human, Mouse, Pig, Rabbit, Rat

Predicted Reactivity: Dog,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IL-1beta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
dot-blot()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-IL-1 beta antibody (ABIN728503)

Chakraborty, Royce, Selomulya, Plebanski: "A novel Approach for Non-Invasive Lung Imaging and Targeting Lung Immune Cells." in: International journal of molecular sciences, Vol. 21, Issue 5, (2020) (PubMed).

Ding, Ren, Yu, Yu, Zhou: "Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice." in: Immunity & ageing : I & A, Vol. 15, pp. 6, (2018) (PubMed).

Liu, Li, Weng: "Effect of BTXA on Inhibiting Hypertrophic Scar Formation in a Rabbit Ear Model." in: Aesthetic plastic surgery, Vol. 41, Issue 3, pp. 721-728, (2017) (PubMed).

Liu, Cao, Li, Wang, Zhang, Dong Zhang, Liu, Yuan, Zhan: "Autophagy induced by DAMPs facilitates the inflammation response in lungs undergoing ischemia-reperfusion injury through promoting TRAF6 ubiquitination." in: Cell death and differentiation, Vol. 24, Issue 4, pp. 683-693, (2017) (PubMed).

Hu, Liu, Deng, Ma, Sheng, Weng, Xu: "Immunoreactivities of IL-1β and IL-1R in oviduct of Chinese brown frog (Rana dybowskii) during pre-hibernation and the breeding period." in: Acta histochemica, Vol. 118, Issue 2, pp. 164-9, (2016) (PubMed).

Du, Niu, Wang, Zhao, Kong, Zhang, Nan: "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." in: Molecular medicine reports, Vol. 13, Issue 3, pp. 2159-66, (2016) (PubMed).

Xi, Hu, Guo, Wang, Sheng, Han, Yuan, Weng, Xu: "Immunoreactivities of NF-κB, IL-1β and IL-1R in the skin of Chinese brown frog (Rana dybowskii)." in: Acta histochemica, Vol. 119, Issue 1, pp. 64-70, (2016) (PubMed).

Heitzer, Kaiser, Kanagaratnam, Zendedel, Hartmann, Beyer, Johann: "Administration of 17β-Estradiol Improves Motoneuron Survival and Down-regulates Inflammasome Activation in Male SOD1(G93A) ALS Mice." in: Molecular neurobiology, (2016) (PubMed).

Han, Lu, Zhao, Wang, Li, Wang: "Electroacupuncture modulated the inflammatory reaction in MCAO rats via inhibiting the TLR4/NF-κB signaling pathway in microglia." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 11199-205, (2016) (PubMed).

Wang, Chen, Li, Ma, Tang: "Anti-inflammatory cytokine TSG-6 inhibits hypertrophic scar formation in a rabbit ear model." in: European journal of pharmacology, Vol. 751, pp. 42-9, (2015) (PubMed).

Johann, Heitzer, Kanagaratnam, Goswami, Rizo, Weis, Troost, Beyer: "NLRP3 inflammasome is expressed by astrocytes in the SOD1 mouse model of ALS and in human sporadic ALS patients." in: Glia, (2015) (PubMed).

Li, Dan, Nie, Hu, Gesang, Bianba, Ze, Ciren: "CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts." in: Molecular medicine reports, Vol. 12, Issue 3, pp. 4332-9, (2015) (PubMed).

de Jesus, Spadacci-Morena, dos Anjos Rabelo, Pinfildi, Fukuda, Plapler: "Low-level laser therapy in IL-1β, COX-2, and PGE2 modulation in partially injured Achilles tendon." in: Lasers in medical science, Vol. 30, Issue 1, pp. 153-8, (2015) (PubMed).

Bagriyanik, Ersoy, Cetinkaya, Ikizoglu, Kutri, Ozcana, Kamanga, Kiray: "The effects of resveratrol on chronic constriction injury of sciatic nerve in rats." in: Neuroscience letters, Vol. 561, pp. 123-7, (2014) (PubMed).

Zhong, Shu, Wang, Luo, Zhong, Ran, Zheng, Yin, Ling: "Enhanced homing of mesenchymal stem cells to the ischemic myocardium by ultrasound-targeted microbubble destruction." in: Ultrasonics, Vol. 52, Issue 2, pp. 281-6, (2011) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1 beta

Background:

Synonyms: IL-1, IL1F2, IL1-BETA, Interleukin-1 beta, IL-1 beta, Catabolin, IL1B

Background: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Gene ID: 3553

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome