Laminin antibody (AA 901-1000)

Catalog No. ABIN728638

Product Details anti-Laminin Antibody

Target: Laminin (LN)

Binding Specificity: AA 901-1000

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Laminin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Laminin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Laminin antibody (ABIN728638)

Miyauchi, Yasuchika, Fukumitsu, Ishii, Ogiso, Minami, Kojima, Yamaoka, Katayama, Kawai, Yoshitoshi-Uebayashi, Kita, Yasuda, Sasaki, Uemoto: "A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells." in: Scientific reports, Vol. 7, Issue 1, pp. 9827, (2017) (PubMed).

Gong, Zhao, Liu, Lu, Liu, Zhou: "A Dynamically Tunable, Bioinspired Micropatterned Surface Regulates Vascular Endothelial and Smooth Muscle Cells Growth at Vascularization." in: Small (Weinheim an der Bergstrasse, Germany), Vol. 12, Issue 41, pp. 5769-5778, (2016) (PubMed).

Su, Zhang, Song, Shi, Fu, Xia, Bai, Hu, Xu, Song, Song: "Tetrachlorobenzoquinone activates nrf2 signaling by keap1 cross-linking and ubiquitin translocation but not keap1-cullin3 complex dissociation." in: Chemical research in toxicology, Vol. 28, Issue 4, pp. 765-74, (2015) (PubMed).

Target Details for Laminin

Target: Laminin (LN)

Alternative Name: laminin (LN Products)

Synonyms: LAMC1 antibody, laminin antibody, laminin, beta 2 (laminin S) antibody, LAMB2 antibody

Background:

Synonyms: CLM, LIS5, Laminin subunit beta-1, Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, LAMB1

Background: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of cerebral cortex. It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons. Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface.

Gene ID: 3912

UniProt: P07942