BDNF antibody (Biotin)

Catalog No. ABIN7630604

The Mouse Monoclonal anti-BDNF antibody has been validated for WB, IHC and ICC. It is suitable to detect BDNF in samples from Human, Rat, Mouse, Pig, Rabbit, Horse and Goat.

Quick Overview for BDNF antibody (Biotin) (ABIN7630604)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivity: Human, Rat, Mouse, Pig, Rabbit, Horse, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This BDNF antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: C3

Product Details anti-BDNF Antibody (Biotin)

Purpose: Biotin-Linked Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

Specificity: The antibody is a mouse monoclonal antibody raised against BDNF. It has been selected for its ability to recognize BDNF in immunohistochemical staining and western blotting.

Cross-Reactivity: Goat, Horse, Human, Mouse, Pig, Rabbit, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: MAA011Mi26Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: Brain Derived Neurotrophic Factor

Background: Neurotrophin, Abrineurin

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity