Insulin antibody (Biotin)

Catalog No. ABIN7631929

This Rabbit Polyclonal antibody specifically detects Insulin in IHC, WB and ICC. It exhibits reactivity toward Human.

Quick Overview for Insulin antibody (Biotin) (ABIN7631929)

Target: Insulin (INS)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-Insulin Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Insulin (INS)

Specificity: The antibody is a rabbit polyclonal antibody raised against INS. It has been selected for its ability to recognize INS in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA448Hu01Polyclonal Antibody to Insulin (INS)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation