CDC42 antibody

Catalog No. ABIN7636484

The Mouse Monoclonal anti-CDC42 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect CDC42 in samples from Human.

Quick Overview for CDC42 antibody (ABIN7636484)

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC42 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CDC42 Antibody

Purpose: Monoclonal Antibody to Cell Division Cycle Protein 42 (CDC42)

Specificity: The antibody is a mouse monoclonal antibody raised against CDC42. It has been selected for its ability to recognize CDC42 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for CDC42

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Alternative Name: CDC42

Background: CDC42Hs, G25K, G25K GTP-binding protein

UniProt: P60953

Pathways: MAPK Signaling, Microtubule Dynamics, RTK Signaling, WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling