SIGLEC8 antibody

Catalog No. ABIN7646144

The Rabbit Polyclonal anti-SIGLEC8 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect SIGLEC8 in samples from Human.

Quick Overview for SIGLEC8 antibody (ABIN7646144)

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SIGLEC8 Antibody

Purpose: Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8)

Specificity: The antibody is a rabbit polyclonal antibody raised against SIGLEC8. It has been selected for its ability to recognize SIGLEC8 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for SIGLEC8

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Alternative Name: SIGLEC8

Background: SAF2, SIGLEC-8, SIGLEC8L, Sialoadhesin family member 2

UniProt: Q9NYZ4